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Validation Data Gallery

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  • Sandwich ELISA standard curve of MP50077-1

    Sandwich ELISA standard curve of MP50077-1

    Sandwich ELISA standard curve of MP50077-1, CD4 Monoclonal Matched Antibody Pair - PBS only. 65147-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Eg0333. 68688-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Sample test of MP50077-1

    Sample test of MP50077-1

    The mean CD4 concentration was determined to be 5.16 ng/mL in HL-60 cell extract based on a 4.3 mg/mL extract load.

  • Sample test of MP50077-1

    Sample test of MP50077-1

    Serum of sixteen individual healthy human donors was measured. The human CD4 concentration of detected samples was determined to be 101.4 pg/mL with a range of 12.7 - 255.0 pg/mL.

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CD4 Monoclonal antibody, PBS Only (Detector)

CD4 Monoclonal Antibody for Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 68688-1-PBS
Clone No.1E11B4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CD4 molecule, CD4mut, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68688-1-PBS targets CD4 as part of a matched antibody pair:

MP50077-1: 65147-1-PBS capture and 68688-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Affinity
KD=1.39 x 10-10M
KOff=4.36 x 10-6M
KOn=3.13 x 104M
Immunogen CD4 fusion protein Eg0333 相同性解析による交差性が予測される生物種
Full Name CD4 molecule
Calculated molecular weight 55 kDa
GenBank accession numberBC025782
Gene Symbol CD4
Gene ID (NCBI) 920
ENSEMBL Gene IDENSG00000010610
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP01730
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.