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Validation Data Gallery

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  • Sandwich ELISA standard curve of MP50077-1

    Sandwich ELISA standard curve of MP50077-1

    Sandwich ELISA standard curve of MP50077-1, CD4 Monoclonal Matched Antibody Pair - PBS only. 65147-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Eg0333. 68688-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Sample test of MP50077-1

    Sample test of MP50077-1

    The mean CD4 concentration was determined to be 5.16 ng/mL in HL-60 cell extract based on a 4.3 mg/mL extract load.

  • Sample test of MP50077-1

    Sample test of MP50077-1

    Serum of sixteen individual healthy human donors was measured. The human CD4 concentration of detected samples was determined to be 101.4 pg/mL with a range of 12.7 - 255.0 pg/mL.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD4 (SK3) (65147-1-Ig)

    FC experiment of human PBMCs using 65147-1-Ig (same clone as 65147-1-PBS)

    1X10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD3 and 0.2 ug Anti-Human CD4 (65147-1-Ig, Clone:SK3) labeled with FlexAble CoraLite® Plus 750 Antibody Labeling Kit for Mouse IgG1 (KFA024) (right), or Mouse IgG1 Isotype Control (left). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65147-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD4 (SK3) (65147-1-Ig)

    FC experiment of human PBMCs using 65147-1-Ig (same clone as 65147-1-PBS)

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD4 (65147-1-Ig, Clone:SK3) labeled with FlexAble CoraLite® Plus 750 Antibody Labeling Kit for Mouse IgG1 (KFA024) (red), or Mouse IgG1 Isotype Control (blue). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65147-1-PBS in a different storage buffer formulation.

Anti-Human CD4 (SK3), PBS Only (Capture)

CD4 Monoclonal Antibody for FC, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 65147-1-PBS
Clone No.SK3

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CD4 molecule, CD4mut, SK3, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65147-1-PBS targets CD4 as part of a matched antibody pair:

MP50077-1: 65147-1-PBS capture and 68688-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Affinity
KD=8.82 x 10-11M
KOff=2.00 x 10-5M
KOn=2.26 x 105M
Immunogen

N/A

相同性解析による交差性が予測される生物種
Full Name CD4 molecule
Calculated molecular weight 55 kDa
GenBank accession numberBC025782
Gene Symbol CD4
Gene ID (NCBI) 920
ENSEMBL Gene IDENSG00000010610
RRIDAB_2918432
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAffinity purification
UNIPROT IDP01730
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).