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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD4 (OKT4) (65134-1-Ig)

    FC experiment of human PBMCs using 65134-1-Ig

    1X10^6 human PBMCs were surface co-stained with CL647 Anti-Human CD3 and 0.2 ug Anti-Human CD4 (65134-1-Ig, Clone:OKT4) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD4 (OKT4) (65134-1-Ig)

    FC experiment of human PBMCs using 65134-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD4 (65134-1-Ig, Clone:OKT4) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

Anti-Human CD4 (OKT4)

CD4 Monoclonal Antibody for FC
Cat No. 65134-1-Ig
Clone No.OKT4

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

Human

Applications

FC

Synonyms

CD4, CD4 molecule, CD4mut

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.2 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65134-1-Ig targets CD4 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen

Human peripheral T cells

相同性解析による交差性が予測される生物種
Full Name CD4 molecule
Calculated molecular weight 55 kDa
GenBank accession numberBC025782
Gene Symbol CD4
Gene ID (NCBI) 920
ENSEMBL Gene IDENSG00000010610
RRIDAB_2881474
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAffinity purification
UNIPROT IDP01730
Storage Buffer PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocols

Product Specific Protocols
FC protocol for CD4 antibody 65134-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols