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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD4 Polyclonal antibody (32406-1-AP)

    WB analysis using 32406-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32406-1-AP (CD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat thymus tissue using CD4 Polyclonal antibody (32406-1-AP)

    IHC staining of rat thymus using 32406-1-AP

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 32406-1-AP (CD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat thymus tissue using CD4 Polyclonal antibody (32406-1-AP)

    IHC staining of rat thymus using 32406-1-AP

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 32406-1-AP (CD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat spleen tissue using CD4 Polyclonal antibody (32406-1-AP)

    IF Staining of rat spleen using 32406-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD4 antibody (32406-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat spleen tissue using CD4 Polyclonal antibody (32406-1-AP)

    IF Staining of rat spleen using 32406-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD4 antibody (32406-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD4 Polyclonal antibody

CD4 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 32406-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

CD4 molecule, p55

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat spleen tissue, rat thymus tissue
Positive IHC detected inrat thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32406-1-AP targets CD4 in WB, IHC, IF-P, ELISA applications and shows reactivity with rat samples.

Tested Reactivity rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Eg2025

Product name: Recombinant Rat CD4 protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 28-394 aa of NM_012705.1

Sequence: KTVVLGKEGGSAELPCESTSRRSASFAWKSSDQKTILGYKNKLLIKGSLELYSRFDSRKNAWERGSFPLIINKLRMEDSQTYVCELENKKEEVELWVFRVTFNPGTRLLQGQSLTLILDSNPKVSDPPIECKHKSSNIVKDSKAFSTHSLRIQDSGIWNCTVTLNQKKHSFDMKLSVLGFASTSITAYKSEGESAEFSFPLNLGEESLQGELRWKAEKAPSSQSWITFSLKNQKVSVQKSTSNPKFQLSETLPLTLQIPQVSLQFAGSGNLTLTLDRGILYQEVNLVVMKVTQPDSNTLTCEVMGPTSPKMRLILKQENQEARVSRQEKVIQVQAPEAGVWQCLLSEGEEVKMDSKIQVLSKGLNQT

相同性解析による交差性が予測される生物種
Full Name Cd4 molecule
Calculated molecular weight51 kDa
Observed molecular weight 51-55 kDa
GenBank accession numberNM_012705.1
Gene Symbol Cd4
Gene ID (NCBI) 24932
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDP05540
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells. CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation. CD4 serves as a receptor for the human immunodeficiency virus (HIV).

Protocols

Product Specific Protocols
IHC protocol for CD4 antibody 32406-1-APDownload protocol
WB protocol for CD4 antibody 32406-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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