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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD36 Recombinant monoclonal antibody (86246-6-RR)

    WB analysis using 86246-6-RR (same clone as 86246-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86246-6-RR (CD36/SR-B3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86246-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using CD36 Recombinant monoclonal antibody (86246-6-RR)

    IHC staining of rat spleen using 86246-6-RR (same clone as 86246-6-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 86246-6-RR (CD36 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86246-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using CD36 Recombinant monoclonal antibody (86246-6-RR)

    IHC staining of rat spleen using 86246-6-RR (same clone as 86246-6-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 86246-6-RR (CD36 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86246-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat liver tissue using CD36 Recombinant monoclonal antibody (86246-6-RR)

    IF Staining of rat liver using 86246-6-RR (same clone as 86246-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using CD36 antibody (86246-6-RR, Clone: 250237G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86246-6-PBS in a different storage buffer formulation.

Rat CD36 Recombinant monoclonal antibody, PBS Only

CD36 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 86246-6-PBS
Clone No.250237G3

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Adipocyte membrane protein, Fat, Fatty acid translocase, Fatty acid transport protein, GP3B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86246-6-PBS targets CD36 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg3222

Product name: Recombinant Rat CD36 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 30-439 aa of

Sequence: GDLLIEKTIKREVVLEEGTIAFKNWVKTGTTVYRQFWIFDVQNPEEVAKNSSKIKVKQRGPYTYRVRYLAKENITQDPKDSTVSFVQPNGAIFEPSLSVGTENDNFTVLNLAVAAAPHIYTNSFVQGVLNSLIKKSKSSMFQTRSLKELLWGYKDPFLSLVPYPISTTVGVFYPYNNTVDGVYKVFNGKDNISKVAIIDTYKGKRNLSYWESYCDMINGTDAASFPPFVEKSQTLRFFSSDICRSIYAVFESEVNLKGIPVYRFVLPANAFASPLQNPDNHCFCTEKVISNNCTSYGVLDIGKCKEGKPVYISLPHFLHASPDVSEPIEGLNPNEDEHRTYLDVEPITGFTLQFAKRLQVNILVKPARKIEALKNLKRPYIVPILWLNETGTIGDEKAEMFRNQVTGKIK

相同性解析による交差性が予測される生物種
Full Name CD36 molecule (thrombospondin receptor)
Calculated molecular weight53 kDa
Observed molecular weight88 kDa
Gene Symbol Cd36
Gene ID (NCBI) 29184
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ07969
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CD36, also named as GP3B and GP4, is a membrane glycoprotein present on platelets, monocytes, erythroid precursors, endothelial cells, and several tumor cell lines. CD36 binds to collagen, thrombospondin, anionic phospholipids, long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport and oxidized LDL. CD36 may function as a cell adhesion molecule. It mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes.

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