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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD36 Polyclonal antibody (32731-1-AP)

    WB analysis using 32731-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32731-1-AP (CD36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD36 Polyclonal antibody (32731-1-AP)

    IHC staining of mouse spleen using 32731-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32731-1-AP (CD36 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD36 Polyclonal antibody (32731-1-AP)

    IHC staining of mouse spleen using 32731-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32731-1-AP (CD36 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CD36 Polyclonal antibody

CD36 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32731-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Glycoprotein IIIb, GPIIIB, GPIV, PAS IV, PAS-4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse spleen tissue, rat spleen tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32731-1-AP targets CD36 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD36 fusion protein Eg3464 相同性解析による交差性が予測される生物種
Full Name CD36 antigen
Calculated molecular weight53 kDa
Observed molecular weight80-85 kDa
GenBank accession numberNM_007643.4
Gene Symbol Cd36
Gene ID (NCBI) 12491
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ08857
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD36, also named as GP3B and GP4, is a membrane glycoprotein present on platelets, monocytes, erythroid precursors, endothelial cells, and several tumor cell lines. CD36 binds to collagen, thrombospondin, anionic phospholipids, long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport and oxidized LDL. CD36 may function as a cell adhesion molecule. It mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Mutation of CD36 will cause platelet glycoprotein IV deficiency which known as CD36 deficiency. Genetic variations in CD36 are associated with susceptibility to coronary heart disease type 7 (CHDS7).

Protocols

Product Specific Protocols
WB protocol for CD36 antibody 32731-1-APDownload protocol
IHC protocol for CD36 antibody 32731-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols