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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cardinal Red™ Anti-Human CD35 (E11)

CD35 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

Cardinal Red™ Fluorescent Dye

CloneNo.

E11

Cat no : CR-65254

Synonyms

C3b/C4b receptor, C3BR, CD35, Complement receptor type 1, CR1, KN

Validation Data Gallery

Applications:
  • FC experiment of human PBMCs using CR-65254

    FC experiment of human PBMCs using CR-65254

    1X10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD35 (CR-65254, Clone:E11) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CR-65254

    FC experiment of human PBMCs using CR-65254

    1X10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD35 (CR-65254, Clone:E11) (red) or Mouse IgG1 Isotype Control (blue). Cells were not fixed. Lymphocytes were gated.

Tested Applications

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CR1/CD35 Ready-To-Use IHC Kit. CR1/CD35 primary antibody included.

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CR-65254 targets CD35 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human CD35 from Acute monocytic leukemia cells and normal blood monocytes 相同性解析による交差性が予測される生物種
Full Name complement component (3b/4b) receptor 1 (Knops blood group)
Calculated molecular weight 224 kDa
GenBank accession numberNM_000573
Gene symbol CR1
Gene ID (NCBI) 1378
Conjugate Cardinal Red™ Fluorescent Dye
Excitation/Emission maxima wavelengths592 nm / 611 nm
Form Liquid
Purification Method N/A
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CD35 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhans cells, and glomerular podocytes (PMID: 19004497). CD35 acts as a receptor for C3b and C4b and plays important roles in the regulation of the complement cascade and clearance of immune complexes.

Protocols

Product Specific Protocols
FC protocol for Cardinal Red™ CD35 antibody CR-65254Download protocol
Standard Protocols
Click here to view our Standard Protocols