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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD31 Recombinant monoclonal antibody (85898-4-RR)

    WB analysis using 85898-4-RR (same clone as 85898-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85898-4-RR (CD31/PECAM-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85898-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat spleen tissue using CD31 Recombinant monoclonal antibody (85898-4-RR)

    WB analysis of rat spleen using 85898-4-RR (same clone as 85898-4-PBS)

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 85898-4-RR (CD31/PECAM-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85898-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using CD31 Recombinant monoclonal antibody (85898-4-RR)

    IHC staining of rat liver using 85898-4-RR (same clone as 85898-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 85898-4-RR (CD31/PECAM-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85898-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using CD31 Recombinant monoclonal antibody (85898-4-RR)

    IHC staining of rat liver using 85898-4-RR (same clone as 85898-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 85898-4-RR (CD31/PECAM-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85898-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using CD31 Recombinant monoclonal antibody (85898-4-RR)

    IHC staining of rat kidney using 85898-4-RR (same clone as 85898-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 85898-4-RR (CD31 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85898-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using CD31 Recombinant monoclonal antibody (85898-4-RR)

    IHC staining of rat kidney using 85898-4-RR (same clone as 85898-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 85898-4-RR (CD31 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85898-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat liver tissue using CD31 Recombinant monoclonal antibody (85898-4-RR)

    IF Staining of rat liver using 85898-4-RR (same clone as 85898-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using CD31 antibody (85898-4-RR, Clone: 250372F8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85898-4-PBS in a different storage buffer formulation.

Rat CD31 Recombinant monoclonal antibody, PBS Only

CD31 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 85898-4-PBS
Clone No.250372F8

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Pecam, Pecam1, PECAM-1, Platelet endothelial cell adhesion molecule

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85898-4-PBS targets CD31 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg1456

Product name: recombinant rat CD31/PECAM-1 protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 18-589 aa of NP_113779.1

Sequence: QENSFTINSIHMESRPSWEVSNGQKLTLQCLVDISTTSKSRPQHQVLFYKDDALVYNVSSSEHTESFVIPQSRVFHAGKYKCTVILNSKEKTTIEYQLTVNGVPMPEVTVDKKEVTEGGIVTVNCSMQEEKPPIYFKIEKVELGTKNVKLSREKTSNMNFVLIEFPIEEQDHLLVFRCQAGVLSGIKMQTSEFIRSEYVTVQEFFSTPKFQIQPPEMIIEGNQLHIKCSVQVAHLAQEFPEIIIQKDKAIVATSKQSKEAVYSVMALVEHSGHYTCKVESNRISKASSILVNITELFPRPKLELSSSRLDQGEMLDLSCSVSGAPVANFTIQKEETVLSQYQNFSKIAEERDSGLYSCTAGIGKVVKRSNLVPVQVCEMLSKPRIFHDAKFEIIKGQIIGISCQSVNGTAPITYRLLRAKSNFQTVQKNSNDPVTFTDKPTRDMEYQCIVDNCHSHPEVRSEILRVKVIAPVDEVTISILSGNDVQSGDEMVLRCSVKEGTGPVTFQFYKEKEGRPFHEETVNDTQVFWHHEQTSKEQEGQYYCTAFNRASIVTSLRSGPLTVRVFLAPWKK

相同性解析による交差性が予測される生物種
Full Name platelet/endothelial cell adhesion molecule 1
Calculated molecular weight76kDa
Observed molecular weight100-130 kDa
GenBank accession numberNP_113779.1
Gene Symbol Pecam1
Gene ID (NCBI) 29583
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ3SWT0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

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