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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD31 Recombinant antibody (84607-4-RR)

    WB analysis using 84607-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84607-4-RR (CD31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD31 Recombinant antibody (84607-4-RR)

    IHC staining of mouse spleen using 84607-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84607-4-RR (CD31 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD31 Recombinant antibody (84607-4-RR)

    IHC staining of mouse spleen using 84607-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84607-4-RR (CD31 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CD31 Recombinant antibody (84607-4-RR)

    IHC staining of mouse brain using 84607-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84607-4-RR (CD31 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CD31 Recombinant antibody (84607-4-RR)

    IHC staining of mouse brain using 84607-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84607-4-RR (CD31 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CD31 Recombinant antibody (84607-4-RR)

    IHC staining of mouse liver using 84607-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84607-4-RR (CD31 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CD31 Recombinant antibody (84607-4-RR)

    IHC staining of mouse liver using 84607-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84607-4-RR (CD31 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of bEnd.3 cells using CD31 Recombinant antibody (84607-4-RR)

    IF Staining of bEnd.3 using 84607-4-RR

    Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CD31/PECAM-1 antibody (84607-4-RR, Clone: 242041E12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84607-4-RR

    Affinity and Kinetic Characterization of 84607-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84607-4-RR against Mouse CD31 were performed. The affinity constant is 0.882 nM.

CD31 Recombinant monoclonal antibody

CD31 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84607-4-RR
Clone No.242041E12

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

242041E12, Pecam, PECAM 1, Pecam1, PECAM-1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inbEnd.3 cells, mouse lung tissue
Positive IHC detected inmouse spleen tissue, mouse brain tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inbEnd.3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84607-4-RR targets CD31 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0846

Product name: Recombinant Mouse CD31/PECAM-1 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 18-590 aa of NM_001305157.1

Sequence: EENSFTINSIHMESLPSWEVMNGQQLTLECLVDISTTSKSRSQHRVLFYKDDAMVYNVTSREHTESYVIPQARVFHSGKYKCTVMLNNKEKTTIEYEVKVHGVSKPKVTLDKKEVTEGGVVTVNCSLQEEKPPIFFKIEKLEVGTKFVKRRIDKTSNENFVLMEFPIEAQDHVLVFRCQAGILSGFKLQESEPIRSEYVTVQESFSTPKFEIKPPGMIIEGDQLHIRCIVQVTHLVQEFTEIIIQKDKAIVATSKQSSEAVYSVMAMVEYSGHYTCKVESNRISKASSIMVNITELFPKPKLEFSSSRLDQGELLDLSCSVSGTPVANFTIQKEETVLSQYQNFSKIAEESDSGEYSCTAGIGKVVKRSGLVPIQVCEMLSKPSIFHDAKSEIIKGHAIGISCQSENGTAPITYHLMKAKSDFQTLEVTSNDPATFTDKPTRDMEYQCRADNCHSHPAVFSEILRVRVIAPVDEVVISILSSNEVQSGSEMVLRCSVKEGTSPITFQFYKEKEDRPFHQAVVNDTQAFWHNKQASKKQEGQYYCTASNRASSMRTSPRSSTLAVRVFLAPWKK

相同性解析による交差性が予測される生物種
Full Name platelet/endothelial cell adhesion molecule 1
Calculated molecular weight81 kDa
Observed molecular weight100-130 kDa
GenBank accession numberNM_001305157.1
Gene Symbol CD31
Gene ID (NCBI) 18613
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ08481-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Protocols

Product Specific Protocols
IF protocol for CD31 antibody 84607-4-RRDownload protocol
IHC protocol for CD31 antibody 84607-4-RRDownload protocol
WB protocol for CD31 antibody 84607-4-RRDownload protocol
Standard Protocols
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