Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | bEnd.3 cells |
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 12 publications below |
| FC | See 1 publications below |
Product Information
65058-1-Ig targets CD31 in IF/ICC, FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
C3H/HeJ mouse hematopoietic progenitor cell line 32D 相同性解析による交差性が予測される生物種 |
| Full Name | platelet/endothelial cell adhesion molecule 1 |
| GenBank accession number | BC008519 |
| Gene Symbol | CD31 |
| Gene ID (NCBI) | 18613 |
| RRID | AB_2918366 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | Q08481 |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD31 antibody 65058-1-Ig | Download protocol |
| IF protocol for CD31 antibody 65058-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Mater Photothermal and Photocatalytic Glycol Chitosan and Polydopamine-Grafted Oxygen Vacancy Bismuth Oxyiodide (BiO1-x I) Nanoparticles for the Diagnosis and Targeted Therapy of Diabetic Wounds | ||
Cell Commun Signal Extracellular vesicles from UTX-knockout endothelial cells boost neural stem cell differentiation in spinal cord injury | ||
Acta Biomater Scaffold-assisted assembly of cell-laden microgels to engineer permeable living constructs for vascularized adipose tissue regeneration. | ||
Phytother Res A new look at angiogenesis inhibition of geniposide in experimental arthritis by blocking angiopoietin-2 exocytosis | ||
Brain Behav Immun Synergistic disruption of the blood-brain barrier in tree shrews by Methamphetamine and HIV-1 Tat via TRPM2-Mediated astrocyte inflammation. | ||
Transl Psychiatry Blood IL-6 is a critical trigger of depressive symptoms in a mouse model for human atopic dermatitis. |