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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD31 Polyclonal antibody (32387-1-AP)

    WB analysis using 32387-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32387-1-AP (CD31 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CD31 Polyclonal antibody (32387-1-AP)

    IHC staining of mouse liver using 32387-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32387-1-AP (CD31 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CD31 Polyclonal antibody (32387-1-AP)

    IHC staining of mouse liver using 32387-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32387-1-AP (CD31 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CD31 Polyclonal antibody (32387-1-AP)

    IHC staining of mouse brain using 32387-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32387-1-AP (CD31 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CD31 Polyclonal antibody (32387-1-AP)

    IHC staining of mouse brain using 32387-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32387-1-AP (CD31 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD31 Polyclonal antibody (32387-1-AP)

    IHC staining of mouse spleen using 32387-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32387-1-AP (CD31 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD31 Polyclonal antibody (32387-1-AP)

    IHC staining of mouse spleen using 32387-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32387-1-AP (CD31 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CD31 Polyclonal antibody

CD31 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32387-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

PECAM-1, Pecam1, PECAM 1, Pecam

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inbEnd.3 cells
Positive IHC detected inmouse liver tissue, mouse brain tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32387-1-AP targets CD31 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name platelet/endothelial cell adhesion molecule 1
Calculated molecular weight81 kDa
Observed molecular weight120 kDa
GenBank accession numberNM_001305157.1
Gene Symbol CD31
Gene ID (NCBI) 18613
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ08481-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD31, also known as PECAM1 (Platelet endothelial cell adhesion molecule), is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. It is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, CD31 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Protocols

Product Specific Protocols
WB protocol for CD31 antibody 32387-1-APDownload protocol
IHC protocol for CD31 antibody 32387-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols