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Validation Data Gallery

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  • Western Blot (WB) analysis of rat liver tissue using CD302 Recombinant antibody (85813-5-RR)

    WB analysis of rat liver using 85813-5-RR (same clone as 85813-5-PBS)

    Rat liver tissue was subjected to SDS PAGE, followed by western blot with 85813-5-RR (Cd302 antibody) at a dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85813-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CD302 Recombinant antibody (85813-5-RR)

    WB analysis using 85813-5-RR (same clone as 85813-5-PBS)

    Mouse liver tissue was subjected to SDS PAGE followed by western blot with 85813-5-RR (Cd302 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85813-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CD302 Recombinant antibody (85813-5-RR)

    IHC staining of mouse liver using 85813-5-RR (same clone as 85813-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85813-5-RR (CD302 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85813-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CD302 Recombinant antibody (85813-5-RR)

    IHC staining of mouse liver using 85813-5-RR (same clone as 85813-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85813-5-RR (CD302 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85813-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD302 Recombinant antibody (85813-5-RR)

    IHC staining of mouse spleen using 85813-5-RR (same clone as 85813-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85813-5-RR (CD302 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85813-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD302 Recombinant antibody (85813-5-RR)

    IHC staining of mouse spleen using 85813-5-RR (same clone as 85813-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85813-5-RR (CD302 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85813-5-PBS in a different storage buffer formulation.

Mouse CD302 Recombinant monoclonal antibody, PBS Only

CD302 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85813-5-PBS
Clone No.242893D7

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

CD302 antigen, Clec13a, C-type lectin domain family 13 member A, Type I transmembrane C-type lectin receptor DCL-1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85813-5-PBS targets CD302 in WB, IHC, Indirect ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg2660

Product name: Recombinant Mouse CD302 protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 21-156 aa of NM_025422.4

Sequence: DCPSSTWVQFQGSCYAFLQVTINVENIEDVRKQCTDHGADMVSIHNEEENAFILDTLQKRWKGPDDLLLGMFYDTDDATFKWYDHSNMTFDKWADQDGEDLVDTCGFLYTKTGEWRKGDCEISSVEGTLCKAANNH

相同性解析による交差性が予測される生物種
Full Name CD302 antigen
Calculated molecular weight24 kDa
Observed molecular weight32 kDa
GenBank accession numberNM_025422.4
Gene Symbol Cd302
Gene ID (NCBI) 66205
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9DCG2-2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CD302 is a C-type lectin receptor involved in cell adhesion and migration, as well as endocytosis and phagocytosis. In mice and humans, CD302 is primarily expressed in the liver, lungs, lymph nodes (LNs), spleen, and bone marrow. In CD302 gene knockout (CD302KO) mice, the migration frequency and number of myeloid dendritic cells (mDC) in CD302KO lymph nodes were reduced compared to the wild type, confirming the functional role of CD302 in mDC migration.

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