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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD300LG Polyclonal antibody

CD300LG Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 15426-1-AP

Synonyms

CD300LG, CLM 9, CLM9, CMRF35 like molecule 9, NEPMUCIN, TREM 4, TREM4

Validation Data Gallery

Applications:
  • WB analysis of human kidney using 15426-1-AP

    WB analysis of human kidney using 15426-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 15426-1-AP (CD300LG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 15426-1-AP

    WB analysis of HepG2 using 15426-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15426-1-AP (CD300LG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 15426-1-AP

    IF Staining of HEK-293 using 15426-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 15426-1-AP (CD300LG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman kidney tissue, HepG2 cells
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15426-1-AP targets CD300LG in WB, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD300LG fusion protein Ag8075 相同性解析による交差性が予測される生物種
Full Name CD300 molecule-like family member g
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC025395
Gene symbol CD300LG
Gene ID (NCBI) 146894
RRIDAB_2074217
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD300LG, also known as CLM9 (CMRF35-like molecule 9), is a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. CD300LG is a receptor which may mediate L-selectin-dependent lymphocyte rollings and may play an important role in inflammation.

Protocols

Product Specific Protocols
WB protocol for CD300LG antibody 15426-1-APDownload protocol
IF protocol for CD300LG antibody 15426-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols