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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using CD300LD Polyclonal antibody (30798-1-AP)

    WB analysis of MCF-7 using 30798-1-AP

    WB result of CD300LD antibody (30798-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD300LD transfected MCF-7 cells.

  • Western Blot (WB) analysis of various lysates using CD300LD Polyclonal antibody (30798-1-AP)

    WB analysis using 30798-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30798-1-AP (CD300LD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  • KD/KO Validated

CD300LD Polyclonal antibody

CD300LD Polyclonal Antibody for WB, ELISA
Cat No. 30798-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CMRF35A4, CMRF35 like molecule 4, CMRF35 A4, CLM 4, CD300D

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, MCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30798-1-AP targets CD300LD in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD300LD fusion protein Ag29307 相同性解析による交差性が予測される生物種
Full Name CD300 molecule-like family member d
Calculated molecular weight 22 kDa
Observed molecular weight40-43 kDa
GenBank accession numberNM_001115152
Gene Symbol CD300LD
Gene ID (NCBI) 100131439
RRIDAB_3669758
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UXZ3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD300LD, also known as CD300D and CMRF35A4, is a member of the CD300 family. The CD300 family of molecules modulates a broad and diverse range of immune cell processes via their paired activating and inhibitory receptor functions. CD300LD is a 194 amino acid single-pass type I membrane protein containing an Ig-like V-type (immunoglobulin-like) domain. The apparent molecular weight is greater than the calculated molecular weight of 22 kDa, probably due to post-translational modification, presumably by glycosylation.

Protocols

Product Specific Protocols
WB protocol for CD300LD antibody 30798-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols