Validation Data Gallery
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Tested Applications
| Positive WB detected in | THP-1 cells, HL-60 cells, human spleen tissue, Jurkat cells, K-562 cells, Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67242-1-Ig targets CD300a in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5776 Product name: Recombinant human CD300A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 21-141 aa of BC032352 Sequence: CRTVAGPVGGSLSVQCPYEKEHRTLNKYWCRPPQIFLCDKIVETKGSAGKRNGRVSIRDSPANLSFTVTLENLTEEDAGTYWCGVDTPWLRDFHDPVVEVEVSVFPASTSMTPASITAAKT 相同性解析による交差性が予測される生物種 |
| Full Name | CD300a molecule |
| Calculated molecular weight | 299 aa, 33 kDa |
| Observed molecular weight | 50-60 kDa |
| GenBank accession number | BC032352 |
| Gene Symbol | CD300a |
| Gene ID (NCBI) | 11314 |
| RRID | AB_2882521 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9UGN4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD300a antibody 67242-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |