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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-12 cells using CD30/TNFRSF8 Recombinant monoclonal antibody (87467-3-RR)

    WB analysis of PC-12 using 87467-3-RR

    PC-12 cells were subjected to SDS PAGE followed by western blot with 87467-3-RR (CD30/TNFRSF8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CD30/TNFRSF8 Recombinant monoclonal antibody (87467-3-RR)

    WB analysis using 87467-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87467-3-RR (CD30/TNFRSF8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using CD30/TNFRSF8 Recombinant monoclonal antibody (87467-3-RR)

    IF Staining of PC-12 using 87467-3-RR

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using CD30/TNFRSF8 antibody (87467-3-RR, Clone: 252212B8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 87467-3-RR

    Affinity of 87467-3-RR

    Biolayer interferometry (BLl) kinetic assays of 87467-3-RR against Rat CD30/TNFRSF8 were performed. The affinity constant is 6.23 nM.

CD30/TNFRSF8 Recombinant monoclonal antibody

CD30/TNFRSF8 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87467-3-RR
Clone No.252212B8

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

CD30, CD30L receptor, Lymphocyte activation antigen CD30, TNFRSF8, Tumor necrosis factor receptor superfamily member 8

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-12 cells, rat spleen tissue, rat thymus tissue, rat lung tissue, mouse spleen tissue
Positive IF/ICC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87467-3-RR targets CD30/TNFRSF8 in WB, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg6226

Product name: Recombinant Rat CD30/TNFRSF8 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 19-282 aa of /

Sequence: FPKDRPLDTTCTGDLSYYPGEAARNCCYQCPSGLSPTQPCPQGPATARSSVILTTTNEDRKCTACVTCLPGLVEKAPCSGNSPRICECQPGMYCSTPAVNSCARCSEKTVVKFPDTAEKNTICDLPSPGSGPNGSNPDDCKTLTSHTTPQAIPTLESPANDSVRSLLPKQVTDFVNEGATKLVKVPESSSSKASMPSPDPGNAEMNMTLKLPPPGTVPDISTSENSMEPASTASTLSLLVDARTSSRMQPTSPLSTGTPFLDPG

相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 8
Calculated molecular weight53 kDa
Observed molecular weight160 kDa
GenBank accession number/
Gene Symbol Tnfrsf8
Gene ID (NCBI) 25069
RRIDAB_3745565
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP97525
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD30, also known as TNFRSF8 or Ki-1, is a transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily. It is composed of an extracellular domain with six cysteine-rich repeats, a transmembrane segment, and an intracellular domain (PMID: 1310894). CD30 is expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin's lymphoma and anaplastic large cell lymphoma (PMID: 9826579; 28885612).

Protocols

Product Specific Protocols
IF protocol for CD30/TNFRSF8 antibody 87467-3-RRDownload protocol
WB protocol for CD30/TNFRSF8 antibody 87467-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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