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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD3 epsilon/CD3e Polyclonal antibody (31889-1-AP)

    WB analysis using 31889-1-AP

    Rat thymus tissue was subjected to SDS PAGE followed by western blot with 31889-1-AP (CD3 epsilon/CD3e antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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CD3 epsilon Polyclonal antibody

CD3 epsilon Polyclonal Antibody for WB, ELISA
Cat No. 31889-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inrat thymus tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31889-1-AP targets CD3 epsilon in WB, ELISA applications and shows reactivity with rat samples.

Tested Reactivity rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD3 molecule, epsilon polypeptide
Calculated molecular weight22kDa
Observed molecular weight25 kDa
GenBank accession numberNP_001101610.2
Gene Symbol Cd3e
Gene ID (NCBI) 315609
RRIDAB_3670133
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657). This polyclonal antibody was raised against the epsilon chain of rat CD3 molecule.

Protocols

Product Specific Protocols
WB protocol for CD3 epsilon antibody 31889-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols