Validation Data Gallery
Tested Applications
| Positive WB detected in | rat heart tissue, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33949-1-AP targets CD297/ART4 in WB, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Eg6315 Product name: Recombinant mouse CD297/ART4 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 24-263 aa of NM_026639.2 Sequence: GSTEAPLKVDVDLTPDSFDDQYQGCSEQMVEELNQGDYFIKEVDTHKYYSRAWQKAHLTWLNQAKALPESMTPVHAVAIVVFTLNLNVSSDLAKAMARAAGSPGQYSQSFHFKYLHYYLTSAIQLLRKDSSTKNGSLCYKVYHGMKDVSIGANVGSTIRFGQFLSASLLKEETRVSGNQTLFTIFTCLGASVQDFSLRKEVLIPPYELFEVVSKSGSPKGDLINLRSAGNMSTYNCQLLK 相同性解析による交差性が予測される生物種 |
| Full Name | ADP-ribosyltransferase 4 |
| Calculated molecular weight | 33 kDa |
| Observed molecular weight | 58 kDa |
| GenBank accession number | NM_026639.2 |
| Gene Symbol | Art4 |
| Gene ID (NCBI) | 109978 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9CRA0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD297/ART4 is a GPI-anchored ecto-ADP-ribosyltransferase best known as the molecular carrier of Dombrock blood group antigens on erythrocytes. Expressed on red blood cells, hematopoietic progenitors, and select immune cells, it participates in cell-surface signaling and immune recognition, though its catalytic activity is limited. CD297/ART4 polymorphisms underlie clinically significant transfusion reactions, making it a key target in hematology, transfusion medicine, and erythroid biology.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD297/ART4 antibody 33949-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |