APC Anti-Mouse CD274 (PD-L1, B7-H1) (10F.9G2)
CD274 (PD-L1, B7-H1) Monoclonal Antibody for FC
Host / Isotype
Rat / IgG2b, kappa
Reactivity
Mouse
Applications
FC
Conjugate
APC Fluorescent Dye
CloneNo.
10F.9G2
Cat no : APC-65073
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC detected in | Anti-CD3/CD28 treated 2 days BALB/C mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| FC | See 1 publications below |
Product Information
APC-65073 targets CD274 (PD-L1, B7-H1) in FC applications and shows reactivity with Mouse samples.
| Tested Reactivity | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | N/A 相同性解析による交差性が予測される生物種 |
| Full Name | CD274 antigen |
| GenBank accession number | BC066841 |
| Gene symbol | Cd274 |
| Gene ID (NCBI) | 60533 |
| RRID | AB_2882973 |
| Conjugate | APC Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 650 nm / 660 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for APC CD274 (PD-L1, B7-H1) antibody APC-65073 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Signal Cancer-associated fibroblasts-induced remodeling of tumor immune microenvironment via Jagged1 in glioma |
