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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD27 Recombinant antibody (85066-5-RR)

    WB analysis using 85066-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85066-5-RR (CD27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD27 Recombinant antibody (85066-5-RR)

    IF Staining of human tonsillitis using 85066-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD27 antibody (85066-5-RR, Clone: 242731F2 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD27 Recombinant antibody (85066-5-RR)

    IF Staining of human tonsillitis using 85066-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD27 antibody (85066-5-RR, Clone: 242731F2 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Ramos cells using CD27 Recombinant antibody (85066-5-RR)

    IF Staining of Ramos using 85066-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Ramos cells using CD27 antibody (85066-5-RR, Clone: 242731F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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CD27 Recombinant antibody

CD27 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IF-P, ELISA
Cat No. 85066-5-RR
Clone No.242731F2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IF-P, ELISA

Synonyms

T cell activation antigen CD27, S152, CD27L receptor, CD27 molecule, CD27 antigen

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRamos cells
Positive IF-P detected inhuman tonsillitis tissue
Positive IF/ICC detected inRamos cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)-PIF-P : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85066-5-RR targets CD27 in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD27 molecule
Calculated molecular weight 29 kDa
Observed molecular weight50 kDa
GenBank accession numberBC012160
Gene Symbol CD27
Gene ID (NCBI) 939
ENSEMBL Gene IDENSG00000139193
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26842
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.

Protocols

Product Specific Protocols
WB protocol for CD27 antibody 85066-5-RRDownload protocol
IF protocol for CD27 antibody 85066-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols