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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD235a (HIR2)

CD235a Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG2b

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

HIR2

Cat no : 65174-1-Ig

Synonyms

CD235a, Glycophorin A, GPA, GPErik, GpMiIII, GPSAT, GYPA, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MN sialoglycoprotein, MNS, PAS 2, Sialoglycoprotein alpha

Validation Data Gallery

Applications:
  • FC experiment of human red blood cells using 65174-1-Ig

    FC experiment of human red blood cells using 65174-1-Ig

    Human red blood cells were surface stained with Anti-Human CD235a (65174-1-Ig, Clone:HIR2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (green), or stained with Mouse IgG2b Isotype Control Antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (black). Cells were not fixed.

Tested Applications

Positive FC detected inhuman red blood cells
Planning an IHC experiment? We recommend our IHCeasy Glycophorin A Ready-To-Use IHC Kit. Glycophorin A primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 555 and CoraLite® Plus 647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65174-1-Ig targets CD235a in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / Mouse IgG2b
Class Monoclonal
Type Antibody
Immunogen Synthetic peptide (Human, N-terminal) 相同性解析による交差性が予測される生物種
Full Name glycophorin A (MNS blood group)
Calculated molecular weight 150 aa, 16 kDa
GenBank accession numberBC005319
Gene symbol GYPA
Gene ID (NCBI) 2993
RRIDAB_2918449
Conjugate Unconjugated
Form Liquid
Purification Method Purified by protein-A affinity chromatography
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C.

Background Information

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)

Protocols

Product Specific Protocols
FC protocol for CD235a antibody 65174-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols