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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse spleen tissue using CD23/Fc epsilon RII Recombinant antibody (84212-3-RR)

    WB analysis of mouse spleen using 84212-3-RR

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 84212-3-RR (CD23/Fc epsilon RII antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD23/Fc epsilon RII Recombinant antibody (84212-3-RR)

    IHC staining of mouse spleen using 84212-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84212-3-RR (CD23/Fc epsilon RII antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD23/Fc epsilon RII Recombinant antibody (84212-3-RR)

    IHC staining of mouse spleen using 84212-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84212-3-RR (CD23/Fc epsilon RII antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84212-3-RR

    Affinity and Kinetic Characterization of 84212-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84212-3-RR against Mouse CD23/Fc epsilon RII were performed. The affinity constant is 0.168 nM.

CD23 Recombinant monoclonal antibody

CD23 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 84212-3-RR
Clone No.241385C9

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

241385C9, Fc-epsilon-RII, Fcer2, Fcer2a, Low affinity immunoglobulin epsilon Fc receptor

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse spleen tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84212-3-RR targets CD23 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg1295

Product name: Recombinant Mouse CD23 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: N-6*His

Domain: 51-331 aa of EDL21948.1

Sequence: TEKNLKQLGDTAIQNVSHVTKDLQKFQSNQLAQKSQVVQMSQNLQELQAEQKQMKAQDSRLSQNLTGLQEDLRNAQSQNSKLSQNLNRLQDDLVNIKSLGLNEKRTASDSLEKLQEEVAKLWIEILISKGTACNICPKNWLHFQQKCYYFGKGSKQWIQARFACSDLQGRLVSIHSQKEQDFLMQHINKKDSWIGLQDLNMEGEFVWSDGSPVGYSNWNPGEPNNGGQGEDCVMMRGSGQWNDAFCRSYLDAWVCEQLATCEISAPLASVTPTRPTPKSEP

相同性解析による交差性が予測される生物種
Full Name Fc receptor, IgE, low affinity II, alpha polypeptide
Calculated molecular weight38kDa
Observed molecular weight46 kDa
GenBank accession numberEDL21948.1
Gene Symbol Fcer2a
Gene ID (NCBI) 14128
RRIDAB_3671769
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP20693-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD23/Fc ε RII) is a low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21, involved in B cell differentiation and IgE regulation. Two forms of Fc ε RII (Fc ε RIIa and Fc ε RIIb) were found to exist, which differ in structure only in the N-terminal cytoplasmic region, and Fc ε RIIa and Fc ε RIIb play roles in effector cells of B-cell and IgE-mediated immunity, respectively. On B cells, initiates IgE-dependent antigen uptake and presentation to T cells. On macrophages, after IgE binding and antigen cross-linking, intracellular killing of parasites is induced by activation of the L-arginine-nitric oxide pathway.

Protocols

Product Specific Protocols
WB protocol for CD23 antibody 84212-3-RRDownload protocol
IHC protocol for CD23 antibody 84212-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols