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Validation Data Gallery

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  • Western Blot (WB) analysis of Ramos cells using CD22 Monoclonal antibody (66103-1-Ig)

    WB analysis of Ramos using 66103-1-Ig (same clone as 66103-1-PBS)

    Ramos, Daudi, and Raji cells were subjected to SDS PAGE followed by western blot with 66103-1-Ig (CD22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using CD22 Monoclonal antibody (66103-1-Ig)

    WB analysis of Raji using 66103-1-Ig (same clone as 66103-1-PBS)

    Untreated and PNGase F-treated lysates of Raji cells were subjected to SDS PAGE followed by western blot with 66103-1-Ig (CD22 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD22 Monoclonal antibody (66103-1-Ig)

    IHC staining of human tonsillitis using 66103-1-Ig (same clone as 66103-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66103-1-Ig (CD22 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD22 Monoclonal antibody (66103-1-Ig)

    IHC staining of human tonsillitis using 66103-1-Ig (same clone as 66103-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66103-1-Ig (CD22 antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD22 Monoclonal antibody (66103-1-Ig)

    IHC staining of human tonsillitis using 66103-1-Ig (same clone as 66103-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66103-1-Ig (CD22 antibody) at dilution of 1:20000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD22 Monoclonal antibody (66103-1-Ig)

    IHC staining of human appendicitis using 66103-1-Ig (same clone as 66103-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66103-1-Ig (CD22 antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD22 Monoclonal antibody (66103-1-Ig)

    IHC staining of human appendicitis using 66103-1-Ig (same clone as 66103-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66103-1-Ig (CD22 antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD22 Monoclonal antibody (66103-1-Ig)

    IF Staining of human tonsillitis using 66103-1-Ig (same clone as 66103-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD22 antibody (66103-1-Ig, Clone: 7A2F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD22 Monoclonal antibody (66103-1-Ig)

    IF Staining of human tonsillitis using 66103-1-Ig (same clone as 66103-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD22 antibody (66103-1-Ig, Clone: 7A2F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using CD22 Monoclonal antibody (66103-1-Ig)

    IF Staining of Raji using 66103-1-Ig (same clone as 66103-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD22 antibody (66103-1-Ig, Clone: 7A2F1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66103-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50311-4

    Cytometric bead array standard curve of MP50311-4

    Cytometric bead array standard curve of MP50311-4, CD22 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66103-1-PBS. Detection antibody: 66103-6-PBS. Standard:Ag17986. Range: 0.781-100 ng/mL.

CD22 Monoclonal antibody, PBS Only (Capture)

CD22 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 66103-1-PBS
Clone No.7A2F1

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Synonyms

7A2F1, B cell receptor CD22, B-cell receptor CD22, BL-CAM, B-lymphocyte cell adhesion molecule

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66103-1-PBS targets CD22 as part of a matched antibody pair:

MP50311-4: 66103-1-PBS capture and 66103-6-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag17986

Product name: Recombinant human CD22 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 166-515 aa of BC109306

Sequence: ILHSPAVEGSQVEFLCMSLANPLPTNYTWYHNGKEMQGRTEEKVHIPKILPWHAGTYSCVAENILGTGQRGPGAELDVQYPPKKVTTVIQNPMPIREGDTVTLSCNYNSSNPSVTRYEWKPHGAWEEPSLGVLKIQNVGWDNTTIACAACNSWCSWASPVALNVQYAPRDVRVRKIKPLSEIHSGNSVSLQCDFSSSHPKEVQFFWEKNGRLLGKESQLNFDSISPEDAGSYSCWVNNSIGQTASKAWTLEVLYAPRRLRVSMSPGDQVMEGKSATLTCESDANPPVSHYTWFDWNNQSLPYHSQKLRLEPVKVQHSGAYWCQGTNSVGKGRSPLSTLTVYYSPETIGRR

相同性解析による交差性が予測される生物種
Full Name CD22 molecule
Calculated molecular weight 847 aa, 95 kDa
Observed molecular weight 135 kDa
GenBank accession numberBC109306
Gene Symbol CD22
Gene ID (NCBI) 933
RRIDAB_2881502
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP20273
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.