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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD22 Polyclonal antibody (32183-1-AP)

    WB analysis using 32183-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32183-1-AP (CD22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CD22 Polyclonal antibody (32183-1-AP)

    IF Staining of mouse spleen using 32183-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD22 antibody (32183-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of mouse splenocytes using CD22 Polyclonal antibody (32183-1-AP)

    FC experiment of mouse splenocytes using 32183-1-AP

    1x10^6 mouse splenocytes were surface stained with 0.25 ug CD22 Polyclonal antibody (32183-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug (CL647-65290), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were not fixed.

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CD22 Polyclonal antibody

CD22 Polyclonal Antibody for WB, IF-P, FC, ELISA
Cat No. 32183-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF-P, FC, ELISA

Synonyms

Siglec2, Sialic acid-binding Ig-like lectin 2, Lyb-8, B-lymphocyte cell adhesion molecule, BL-CAM

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse spleen tissue
Positive IF-P detected inmouse spleen tissue
Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32183-1-AP targets CD22 in WB, IF-P, FC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD22 antigen
Calculated molecular weight97kDa
Observed molecular weight 120-150 kDa
GenBank accession numberNM_001043317.2
Gene Symbol Cd22
Gene ID (NCBI) 12483
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP35329-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.

Protocols

Product Specific Protocols
WB protocol for CD22 antibody 32183-1-APDownload protocol
IF protocol for CD22 antibody 32183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols