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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD22 Polyclonal antibody

CD22 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30954-1-AP

Synonyms

B cell receptor CD22, BL CAM, CD22, CD22 molecule, FLJ22814, SIGLEC 2, SIGLEC2, T cell surface antigen Leu 14

Validation Data Gallery

Applications:
  • WB analysis using 30954-1-AP

    WB analysis using 30954-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30954-1-AP (CD22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inDaudi cells, Raji cells, Ramos cells
Planning an IHC experiment? We recommend our IHCeasy CD22 Ready-To-Use IHC Kit. CD22 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Sample-dependent, check data in validation data gallery

Product Information

30954-1-AP targets CD22 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD22 molecule
Calculated molecular weight95kd
Observed molecular weight120-130 kDa
GenBank accession numberNM_001771.4
Gene symbol CD22
Gene ID (NCBI) 933
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.

Protocols

Product Specific Protocols
WB protocol for CD22 antibody 30954-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols