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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD200 Recombinant antibody (83437-5-RR)

    WB analysis using 83437-5-RR (same clone as 83437-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83437-5-RR (CD200 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83437-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CD200 Recombinant antibody (83437-5-RR)

    IHC staining of mouse brain using 83437-5-RR (same clone as 83437-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83437-5-RR (CD200 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83437-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CD200 Recombinant antibody (83437-5-RR)

    IHC staining of mouse brain using 83437-5-RR (same clone as 83437-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83437-5-RR (CD200 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83437-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using CD200 Recombinant antibody (83437-5-RR)

    IHC staining of mouse cerebellum using 83437-5-RR (same clone as 83437-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83437-5-RR (CD200 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83437-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using CD200 Recombinant antibody (83437-5-RR)

    IHC staining of mouse cerebellum using 83437-5-RR (same clone as 83437-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83437-5-RR (CD200 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83437-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83437-5-RR

    Affinity and Kinetic Characterization of 83437-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83437-5-RR against Mouse CD200 were performed. The affinity constant is 28.8 nM.

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Mouse CD200 Recombinant antibody, PBS Only

CD200 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83437-5-PBS
Clone No.240391A8

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

OX2, Mox2, CD200 antigen, 240391A8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83437-5-PBS targets CD200 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD200 antigen
Calculated molecular weight31 kDa
Gene Symbol Cd200
Gene ID (NCBI) 17470
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO54901
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).