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- KD/KO Validated
CD200 Monoclonal antibody
CD200 Monoclonal Antibody for WB, FC, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IF, FC, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
5F3D6
Cat no : 66282-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | fetal human brain tissue, human spleen tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | U-937 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
| IHC | See 2 publications below |
| IF | See 3 publications below |
Product Information
66282-1-Ig targets CD200 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD200 fusion protein Ag5045 相同性解析による交差性が予測される生物種 |
| Full Name | CD200 molecule |
| Calculated molecular weight | 269 aa, 30 kDa |
| Observed molecular weight | 40-50 kDa |
| GenBank accession number | BC022522 |
| Gene symbol | CD200 |
| Gene ID (NCBI) | 4345 |
| RRID | AB_2881665 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD200 antibody 66282-1-Ig | Download protocol |
| IHC protocol for CD200 antibody 66282-1-Ig | Download protocol |
| FC protocol for CD200 antibody 66282-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancers (Basel) Multiplex Quantitative Analysis of Tumor-Infiltrating Lymphocytes, Cancer-Associated Fibroblasts, and CD200 in Pancreatic Cancer. | ||
Sci Rep CD200-positive cancer associated fibroblasts augment the sensitivity of Epidermal Growth Factor Receptor mutation-positive lung adenocarcinomas to EGFR Tyrosine kinase inhibitors.
| ||
Oncoimmunology CD200 and CD200R1 are differentially expressed and have differential prognostic roles in non-small cell lung cancer.
|
