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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD20 Monoclonal antibody, PBS Only

CD20 Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4A7G3

Cat no : 60271-1-PBS

Synonyms

B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7

Validation Data Gallery

Applications:
  • WB analysis using 60271-1-Ig (same clone as 60271-1-PBS)

    WB analysis using 60271-1-Ig (same clone as 60271-1-PBS)

    Raji cells (CD20+), Daudi cells (CD20+), Ramos cells (CD20+), Jurkat cells (CD20-), and HeLa cells (CD20-) were subjected to SDS PAGE followed by western blot with 60271-1-Ig (CD20 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    IHC staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60271-1-Ig (CD20 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD20 antibody included
  • IHC staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    IHC staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60271-1-Ig (CD20 antibody) at dilution of 1:12800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD20 antibody included
  • IHC staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    IHC staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60271-1-Ig (CD20 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD20 antibody included
  • IHC staining of human appendicitis using 60271-1-Ig (same clone as 60271-1-PBS)

    IHC staining of human appendicitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60271-1-Ig (CD20 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD20 antibody included
  • IHC staining of human appendicitis using 60271-1-Ig (same clone as 60271-1-PBS)

    IHC staining of human appendicitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60271-1-Ig (CD20 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD20 antibody included
  • IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (60271-1-Ig, Clone: 4A7G3 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CD3 antibody (17617-1-AP, green). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (60271-1-Ig, Clone: 4A7G3 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CD3 antibody (17617-1-AP, green). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • IF Staining of Raji using 60271-1-Ig (same clone as 60271-1-PBS)

    IF Staining of Raji using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD20 antibody (60271-1-Ig, Clone: 4A7G3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (60271-1-Ig, Clone: 4A7G3 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CD3 antibody (17617-1-AP, green). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60271-1-Ig (CD20 antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60271-1-Ig (CD20 antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 18106-1-AP (PD-1/CD279 rabbit polyclonal antibody) at dilution of 1:100 and 60271-1-Ig (CD20 mouse monoclonal antibody) at dilution of 1:200, further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 18106-1-AP, and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 60271-1-Ig. This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    IF Staining of human tonsillitis using 60271-1-Ig (same clone as 60271-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 18106-1-AP (PD-1/CD279 rabbit polyclonal antibody) at dilution of 1:100 and 60271-1-Ig (CD20 mouse monoclonal antibody) at dilution of 1:200, further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 18106-1-AP, and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 60271-1-Ig. This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

  • FC experiment of Daudi using 60271-1-Ig (same clone as 60271-1-PBS)

    FC experiment of Daudi using 60271-1-Ig (same clone as 60271-1-PBS)

    1X10^6 Daudi cells were intracellularly stained with 0.4 ug Anti-Human CD20 (60271-1-Ig, Clone:4A7G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60271-1-PBS in a different storage buffer formulation.

Tested Applications

Planning an IHC experiment? We recommend our IHCeasy CD20 Ready-To-Use IHC Kit. CD20 primary antibody included.

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

60271-1-PBS targets CD20 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD20 fusion protein Ag17309 相同性解析による交差性が予測される生物種
Full Name membrane-spanning 4-domains, subfamily A, member 1
Calculated molecular weight 297 aa, 33 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC002807
Gene symbol MS4A1
Gene ID (NCBI) 931
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.