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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD2 Recombinant antibody (84360-4-RR)

    WB analysis using 84360-4-RR

    CTLL-2 cells were subjected to SDS PAGE followed by western blot with 84360-4-RR (CD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse thymus tissue using CD2 Recombinant antibody (84360-4-RR)

    IHC staining of mouse thymus using 84360-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 84360-4-RR (CD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse thymus tissue using CD2 Recombinant antibody (84360-4-RR)

    IHC staining of mouse thymus using 84360-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 84360-4-RR (CD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CD2 Recombinant antibody

CD2 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84360-4-RR
Clone No.241548D7

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

T-cell surface antigen CD2, Lymphocyte antigen 37, Ly-37, LFA-3 receptor, LFA-2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCTLL-2 cells
Positive IHC detected inmouse thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84360-4-RR targets CD2 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein 相同性解析による交差性が予測される生物種
Full Name CD2 antigen
Calculated molecular weight38 kDa
Observed molecular weight60 kDa
GenBank accession numberNM_013486.2
Gene Symbol CD2
Gene ID (NCBI) 12481
RRIDAB_3671898
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08920
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

Protocols

Product Specific Protocols
WB protocol for CD2 antibody 84360-4-RRDownload protocol
IHC protocol for CD2 antibody 84360-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols