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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD1e Polyclonal antibody (22463-1-AP)

    WB analysis using 22463-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1e antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using CD1e Polyclonal antibody (22463-1-AP)

    WB analysis of human plasma using 22463-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using CD1e Polyclonal antibody (22463-1-AP)

    WB analysis of A431 using 22463-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CD1e Polyclonal antibody (22463-1-AP)

    WB analysis of Jurkat using 22463-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CD1e Polyclonal antibody (22463-1-AP)

    WB analysis of Jurkat using 22463-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using CD1e Polyclonal antibody (22463-1-AP)

    WB analysis of MCF-7 using 22463-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using CD1e Polyclonal antibody (22463-1-AP)

    WB analysis of NIH/3T3 using 22463-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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CD1e Polyclonal antibody

CD1e Polyclonal Antibody for WB, ELISA
Cat No. 22463-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

R2G1, R2, hCD1e, CD1e molecule

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, MOLT-4 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22463-1-AP targets CD1e in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD1e fusion protein Ag18008 相同性解析による交差性が予測される生物種
Full Name CD1e molecule
Calculated molecular weight 388 aa, 44 kDa
Observed molecular weight51 kDa
GenBank accession numberBC131693
Gene Symbol CD1E
Gene ID (NCBI) 913
RRIDAB_3669398
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP15812
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD1e molecule (CD1e, also known as R2 and CD1A) is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin (PMID: 10948205). The discoverers of the CD1 locus originally separated CD1 proteins into group 1 (CD1a, CD1b, and CD1c) and group 2 (CD1d), based on amino acid sequence homology (PMID: 2467814). CD1e is an important modulator of group 1 and group 2 CD1-restricted responses influencing the lipid antigen availability and the generation and persistence of CD1-lipid complexes (PMID: 21844346).

Protocols

Product Specific Protocols
WB protocol for CD1e antibody 22463-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols