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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD1c Recombinant monoclonal antibody (87145-2-RR)

    WB analysis using 87145-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87145-2-RR (CD1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD1c Recombinant monoclonal antibody (87145-2-RR)

    IHC staining of human tonsillitis using 87145-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87145-2-RR (CD1c antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CD1c Recombinant monoclonal antibody (87145-2-RR)

    IF Staining of Jurkat using 87145-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD1C antibody (87145-2-RR, Clone: 252399G7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 87145-2-RR

    Affinity of 87145-2-RR

    Biolayer interferometry (BLl) kinetic assays of 87145-2-RR against Human CD1c were performed. The affinity constant is 15.7 nM.

CD1c Recombinant monoclonal antibody

CD1c Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 87145-2-RR
Clone No.252399G7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

BDCA1, CD1, CD1c molecule, R7, T-cell surface glycoprotein CD1c

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, MOLT-4 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87145-2-RR targets CD1c in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg9187

Product name: Recombinant human B2M-CD1C protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-Twin-Strep-Tag

Domain: 18-333 aa of

Sequence: NADASQEHVSFHVIQIFSFVNQSWARGQGSGWLDELQTHGWDSESGTIIFLHNWSKGNFSNEELSDLELLFRFYLFGLTREIQDHASQDYSKYPFEVQVKAGCELHSGKSPEGFFQVAFNGLDLLSFQNTTWVPSPGCGSLAQSVCHLLNHQYEGVTETVYNLIRSTCPRFLLGLLDAGKMYVHRQVRPEAWLSSRPSLGSGQLLLVCHASGFYPKPVWVTWMRNEQEQLGTKHGDILPNADGTWYLQVILEVASEEPAGLSCRVRHSSLGGQDIILYWGHHFSM

相同性解析による交差性が予測される生物種
Full Name CD1c molecule
Observed molecular weight38 kDa
Gene Symbol CD1C
Gene ID (NCBI) 911
RRIDAB_3745368
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP29017
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD1c molecule (CD1c, also known as R7, CD1, CD1A, and BDCA1) is a member of the CD1 family of transmembrane glycoproteins, which is synthesized in the endoplasmic reticulum and expressed on the monocytes, marginal zone B cells in lymph nodes as well as peripheral blood (PMID: 23468110). The CD1 proteins belong to the family of major histocompatibility complex (MHC) class I-like proteins that present lipid-based antigens to T cells (PMID: 15032598; 23127489). CD1c shows interaction with both αβ or γδ T cells and mediates T cell responses against mycobacterium tuberculosis (PMID: 10727456; 10786796).

Protocols

Product Specific Protocols
IF protocol for CD1c antibody 87145-2-RRDownload protocol
IHC protocol for CD1c antibody 87145-2-RRDownload protocol
WB protocol for CD1c antibody 87145-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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