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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD1c Polyclonal antibody (28838-1-AP)

    IHC staining of human tonsillitis using 28838-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28838-1-AP (CD1c antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD1c Polyclonal antibody (28838-1-AP)

    IHC staining of human tonsillitis using 28838-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28838-1-AP (CD1C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD1c Polyclonal antibody

CD1c Polyclonal Antibody for IHC, ELISA
Cat No. 28838-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

T-cell surface glycoprotein CD1c, R7, CD1c molecule, CD1, BDCA1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28838-1-AP targets CD1c in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag29830

Product name: Recombinant human CD1C protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 18-118 aa of BC126467

Sequence: NADASQEHVSFHVIQIFSFVNQSWARGQGSGWLDELQTHGWDSESGTIIFLHNWSKGNFSNEELSDLELLFRFYLFGLTREIQDHASQDYSKYPFEVQVKA

相同性解析による交差性が予測される生物種
Full Name CD1c molecule
Calculated molecular weight 333 aa, 38 kDa
GenBank accession numberBC126467
Gene Symbol CD1C
Gene ID (NCBI) 911
RRIDAB_3086090
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP29017
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD1c molecule (CD1c, also known as R7, CD1, CD1A, and BDCA1) is a member of the CD1 family of transmembrane glycoproteins, which synthesizes in the endoplasmic reticulum and expresses on the monocytes, marginal zone B cells in lymph nodes as well as peripheral blood (PMID: 23468110). The CD1 proteins belong to the family of major histocompatibility complex (MHC) class I-like proteins that present lipid-based antigens to T cells (PMID: 15032598; 23127489). CD1c shows interaction with both αβ or γδ T cells and mediates T cell responses against mycobacterium tuberculosis (PMID: 10727456; 10786796).

Protocols

Product Specific Protocols
IHC protocol for CD1c antibody 28838-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols