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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD19 Recombinant antibody (83254-1-RR)

    WB analysis using 83254-1-RR (same clone as 83254-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83254-1-RR (CD19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD19 Recombinant antibody (83254-1-RR)

    IHC staining of mouse spleen using 83254-1-RR (same clone as 83254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83254-1-RR (CD19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using CD19 Recombinant antibody (83254-1-RR)

    IHC staining of rat spleen using 83254-1-RR (same clone as 83254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 83254-1-RR (CD19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD19 Recombinant antibody (83254-1-RR)

    IHC staining of mouse spleen using 83254-1-RR (same clone as 83254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83254-1-RR (CD19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CD19 Recombinant antibody (83254-1-RR)

    IF Staining of mouse spleen using 83254-1-RR (same clone as 83254-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD19 antibody (83254-1-RR, Clone: 240135A3 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83254-1-RR

    Affinity and Kinetic Characterization of 83254-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83254-1-RR against Mouse CD19 were performed. The affinity constant is 0.303 nM.

Mouse CD19 Recombinant monoclonal antibody, PBS Only

CD19 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 83254-1-PBS
Clone No.240135A3

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Synonyms

240135A3, B-lymphocyte antigen CD19, CD19 antigen

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83254-1-PBS targets CD19 in WB, IHC, IF-P, Cytometric bead array, Indirect ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0907

Product name: Recombinant Mouse CD19 protein (His Tag)

Source: mammalian cells-derived, pCMV (S18)

Tag: C-6*HIS

Domain: 19-287 aa of NM_009844.2

Sequence: RPQKSLLVEVEEGGNVVLPCLPDSSPVSSEKLAWYRGNQSTPFLELSPGSPGLGLHVGSLGILLVIVNVSDHMGGFYLCQKRPPFKDIWQPAWTVNVEDSGEMFRWNASDVRDLDCDLRNRSSGSHRSTSGSQLYVWAKDHPKVWGTKPVCAPRGSSLNQSLINQDLTVAPGSTLWLSCGVPPVPVAKGSISWTHVHPRRPNVSLLSLSLGGEHPVREMWVWGSLLLLPQATALDEGTYYCLRGNLTIERHVKVIARSAVWLWLLRTGG

相同性解析による交差性が予測される生物種
Full Name CD19 antigen
Calculated molecular weight60 kDa
Observed molecular weight62-70 kDa
GenBank accession numberNM_009844.2
Gene Symbol Cd19
Gene ID (NCBI) 12478
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP25918
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CD19 is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).