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Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using CD19 Monoclonal antibody (66298-1-Ig)

    WB analysis of Raji using 66298-1-Ig (same clone as 66298-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66298-1-Ig (CD19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66298-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using CD19 Monoclonal antibody (66298-1-Ig)

    WB analysis of Raji using 66298-1-Ig (same clone as 66298-1-PBS)

    Untreated and PNGase F-treated lysates of Raji cells were subjected to SDS PAGE followed by western blot with 66298-1-Ig (CD19 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66298-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Ramos cells using CD19 Monoclonal antibody (66298-1-Ig)

    WB analysis of Ramos using 66298-1-Ig (same clone as 66298-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 66298-1-Ig (CD19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66298-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using CD19 Monoclonal antibody (66298-1-Ig)

    WB analysis of Daudi using 66298-1-Ig (same clone as 66298-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 66298-1-Ig (CD19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66298-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD19 Monoclonal antibody (66298-1-Ig)

    IHC staining of human tonsillitis using 66298-1-Ig (same clone as 66298-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66298-1-Ig (CD19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66298-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD19 Monoclonal antibody (66298-1-Ig)

    IHC staining of human tonsillitis using 66298-1-Ig (same clone as 66298-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66298-1-Ig (CD19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66298-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD19 Monoclonal antibody (66298-1-Ig)

    IHC staining of human tonsillitis using 66298-1-Ig (same clone as 66298-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66298-1-Ig (CD19 antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66298-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD19 Monoclonal antibody (66298-1-Ig)

    IHC staining of human tonsillitis using 66298-1-Ig (same clone as 66298-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66298-1-Ig (CD19 antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66298-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using CD19 Monoclonal antibody (66298-1-Ig)

    IF Staining of Raji using 66298-1-Ig (same clone as 66298-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD19 antibody (66298-1-Ig, Clone: 1C10A1 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66298-1-PBS in a different storage buffer formulation.

CD19 Monoclonal antibody, PBS Only

CD19 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66298-1-PBS
Clone No.1C10A1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

1C10A1, B lymphocyte antigen CD19, B4, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66298-1-PBS targets CD19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag19945

Product name: Recombinant human CD19 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 21-291 aa of BC006338

Sequence: EEPLVVKVEEGDNAVLQCLKGTSDGPTQQLTWSRESPLKPFLKLSLGLPGLGIHMRPLAIWLFIFNVSQQMGGFYLCQPGPPSEKAWQPGWTVNVEGSGELFRWNVSDLGGLGCGLKNRSSEGPSSPSGKLMSPKLYVWAKDRPEIWEGEPPCLPPRDSLNQSLSQDLTMAPGSTLWLSCGVPPDSVSRGPLSWTHVHPKGPKSLLSLELKDDRPARDMWVMETGLLLPRATAQDAGKYYCHRGNLTMSFHLEITARPVLWHWLLRTGGWK

相同性解析による交差性が予測される生物種
Full Name CD19 molecule
Calculated molecular weight 556 aa, 61 kDa
Observed molecular weight 95 kDa
GenBank accession numberBC006338
Gene Symbol CD19
Gene ID (NCBI) 930
ENSEMBL Gene IDENSG00000177455
RRIDAB_2881681
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP15391
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).

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