Validation Data Gallery
Tested Applications
| Positive WB detected in | A20 cells, RAW 264.7 cells, mouse spleen tissue, mouse thymus tissue |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87481-2-RR targets CD18 in WB, IHC, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg10459 Product name: Recombinant mouse ITB2 protein Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-Twin-Strep-Tag Sequence: \ 相同性解析による交差性が予測される生物種 |
| Full Name | integrin beta 2 |
| Observed molecular weight | 85-90 kDa |
| Gene Symbol | CD18 |
| Gene ID (NCBI) | 16414 |
| RRID | AB_3745573 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during the immune response, and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CD18 antibody 87481-2-RR | Download protocol |
| WB protocol for CD18 antibody 87481-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |