Validation Data Gallery

Filter: AllWBIHCIF-PIF/ICC

WB analysis using 10554-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 10554-1-AP (CD18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human tonsillitis using 10554-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10554-1-AP (CD18 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 10554-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10554-1-AP (CD18 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human tonsillitis using 10554-1-AP
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 10554-1-AP (CD18 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of human tonsillitis using 10554-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD18 antibody (10554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of THP-1 using 10554-1-AP
Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD18 antibody (10554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

CD18 Polyclonal antibody

CD18 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 10554-1-AP

Publications(35)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

ITGB2, Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta, Integrin beta 2, Integrin beta-2, LAD

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (35)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	U-937 cells
Positive IHC detected in	human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human tonsillitis tissue
Positive IF/ICC detected in	THP-1 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 18 publications below
IHC	See 8 publications below
IF	See 11 publications below

Product Information

10554-1-AP targets CD18 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0861 Product name: Recombinant human CD18 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 469-769 aa of BC005861 Sequence: ICRCDTGYIGKNCECQTQGRSSQELEGSCRKDNNSIICSGLGDCVCGQCLCHTSDVPGKLIYGQYCECDTINCERYNGQVCGGPGRGLCFCGKCRCHPGFEGSACQCERTTEGCLNPRRVECSGRGRCRCNVCECHSGYQLPLCQECPGCPSPCGKYISCAECLKFEKGPFGKNCSAACPGLQLSNNPVKGRTCKERDSEGCWVAYTLEQQDGMDRYLIYVDESRECVAGPNIAAIVGGTVAGIVLIGILLLVIWKALIHLSDLREYRRFEKEKLKSQWNNDNPLFKSATTTVMNPKFAES 相同性解析による交差性が予測される生物種
Full Name	integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
Calculated molecular weight	85 kDa
Observed molecular weight	85-90 kDa
GenBank accession number	BC005861
Gene Symbol	CD18
Gene ID (NCBI)	3689
ENSEMBL Gene ID	ENSG00000160255
RRID	AB_2877736
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P05107
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).

Protocols

Product Specific Protocols
IF protocol for CD18 antibody 10554-1-AP	Download protocol
IHC protocol for CD18 antibody 10554-1-AP	Download protocol
WB protocol for CD18 antibody 10554-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human		J Exp Med Layilin augments integrin activation to promote antitumor immunity. Authors - Kelly M Mahuron View Article
pig	IF	Research (Wash D C) Regulating Blood Clot Fibrin Films to Manipulate Biomaterial-Mediated Foreign Body Responses Authors - Yang Zou View Article
human	WB,IF	ACS Appl Mater Interfaces Leukocyte-Mediated Combined Targeted Chemo and Gene Therapy for Esophageal Cancer. Authors - Yali Jun View Article
human	WB,IHC,IF	Theranostics ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system. Authors - Xiaoxin Zhang KD Validated View Article
human	WB	Int J Biol Macromol Diethylaminoethyl-dextran and monocyte cell membrane coated 1,8-cineole delivery system for intracellular delivery and synergistic treatment of atherosclerosis Authors - Feng Jiang View Article
	WB	Cardiovasc Res Myocardin-related transcription factor A (MRTF-A) regulates integrin beta 2 transcription to promote macrophage infiltration and cardiac hypertrophy in mice. Authors - Li Liu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10554-1-AP

IHC staining of human tonsillitis using 10554-1-AP

IHC staining of human tonsillitis using 10554-1-AP

IF Staining of human tonsillitis using 10554-1-AP

IF Staining of human tonsillitis using 10554-1-AP

IF Staining of THP-1 using 10554-1-AP

CD18 Polyclonal antibody

CD18 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Layilin augments integrin activation to promote antitumor immunity.

Regulating Blood Clot Fibrin Films to Manipulate Biomaterial-Mediated Foreign Body Responses

Leukocyte-Mediated Combined Targeted Chemo and Gene Therapy for Esophageal Cancer.

ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system.

Diethylaminoethyl-dextran and monocyte cell membrane coated 1,8-cineole delivery system for intracellular delivery and synergistic treatment of atherosclerosis

Myocardin-related transcription factor A (MRTF-A) regulates integrin beta 2 transcription to promote macrophage infiltration and cardiac hypertrophy in mice.