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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD169 Polyclonal antibody

CD169 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55427-1-AP

Synonyms

CD169, Sialoadhesin, SIGLEC 1, SIGLEC1, SN

Validation Data Gallery

Applications:
  • IHC staining of human liver using 55427-1-AP

    IHC staining of human liver using 55427-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55427-1-AP (CD169 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 55427-1-AP

    IHC staining of human liver using 55427-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55427-1-AP (CD169 Antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human tonsillitis using 55427-1-AP

    IHC staining of human tonsillitis using 55427-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55427-1-AP (CD169 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human tonsillitis using 55427-1-AP

    IHC staining of human tonsillitis using 55427-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55427-1-AP (CD169 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of human tonsillitis using 55427-1-AP

    IF Staining of human tonsillitis using 55427-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD169 antibody (55427-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human tonsillitis using 55427-1-AP

    IF Staining of human tonsillitis using 55427-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD169 antibody (55427-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inhuman liver tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

55427-1-AP targets CD169 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymonkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name sialic acid binding Ig-like lectin 1, sialoadhesin
Calculated molecular weight 183 kDa
GenBank accession numberNM_023068
Gene symbol SIGLEC1
Gene ID (NCBI) 6614
RRIDAB_2881327
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for CD169 antibody 55427-1-APDownload protocol
IF protocol for CD169 antibody 55427-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
monkeyWB

BMC Vet Res

Curcumin is a promising inhibitor of genotype 2 porcine reproductive and respiratory syndrome virus infection.

Authors - Taofeng Du
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Emma (Verified Customer) (02-22-2022)

Works well by IF on FFPE prostate tissue. (Tris-EDTA antigen retrieval).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:25
  • Cell Tissue Type: Prostate