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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using CD164 Recombinant monoclonal antibody (85723-4-RR)

    WB analysis of mouse liver using 85723-4-RR (same clone as 85723-4-PBS)

    mouse liver tissue was subjected to SDS PAGE followed by western blot with 85723-4-RR (CD164 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85723-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CD164 Recombinant monoclonal antibody (85723-4-RR)

    IHC staining of mouse kidney using 85723-4-RR (same clone as 85723-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85723-4-RR (CD164 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85723-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RM-1 cells using CD164 Recombinant monoclonal antibody (85723-4-RR)

    IF Staining of RM-1 using 85723-4-RR (same clone as 85723-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RM-1 cells using CD164 antibody (85723-4-RR, Clone: 242986G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85723-4-PBS in a different storage buffer formulation.

Mouse CD164 Recombinant monoclonal antibody, PBS Only

CD164 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85723-4-PBS
Clone No.242986G5

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Endolyn, MGC-24, MGC-24v, MUC-24, Multi-glycosylated core protein 24

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85723-4-PBS targets CD164 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg2645

Product name: Recombinant Mouse CD164 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 24-162 aa of NM_016898.2

Sequence: QPNITTLAPNVTEVPTTTTKVVPTTQMPTVLPETCASFNSCVSCVNATFTNNITCFWLHCQEANKTYCANEPLSNCSQVNRTDLCSVIPPTTPVPTNSTAKPTTRPSSPTPTPSVVTSAGTTNTTLTPTSQPERKSTFD

相同性解析による交差性が予測される生物種
Full Name CD164 antigen
Calculated molecular weight21 kDa
Observed molecular weight80 kDa
GenBank accession numberNM_016898.2
Gene Symbol Cd164
Gene ID (NCBI) 53599
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9R0L9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Sialomucins are a heterogeneous group of secreted or membrane-associated mucins that appear to play 2 key but opposing roles in vivo: first as cytoprotective or antiadhesive agents, and second as adhesion receptors. CD164 is a type I integral transmembrane sialomucin that functions as an adhesion receptor (PMID: 9680353)(PMID: 17077324). Sialomucin CD164 (MUC-24), also referred to multi-glycosylated core protein 24 (MGC24), is known to function as a receptor that regulates stem cell localization to the bone marrow. CD164 may play a key role in hematopoiesis by facilitating the adhesion of CD34+ cells to the stroma and by negatively regulating CD34+CD38(lo/-) cell proliferation. Important role of CD164 in in prostate cancer metastasis, promoting myogenesis and regulating myoblast migration so far have been revealed (PMID: 9763543)(PMID: 16859559)(PMID: 17077324).

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