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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD163 Recombinant antibody (83285-4-RR)

    WB analysis using 83285-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83285-4-RR (CD163 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using CD163 Recombinant antibody (83285-4-RR)

    WB analysis of mouse lung using 83285-4-RR

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 83285-4-RR (CD163 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat spleen tissue using CD163 Recombinant antibody (83285-4-RR)

    WB analysis of rat spleen using 83285-4-RR

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 83285-4-RR (CD163 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CD163 Recombinant antibody (83285-4-RR)

    IHC staining of mouse liver using 83285-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83285-4-RR (CD163 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CD163 Recombinant antibody (83285-4-RR)

    IHC staining of mouse liver using 83285-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83285-4-RR (CD163 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD163 Recombinant antibody (83285-4-RR)

    IHC staining of mouse spleen using 83285-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83285-4-RR (CD163 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD163 Recombinant antibody (83285-4-RR)

    IHC staining of mouse spleen using 83285-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83285-4-RR (CD163 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat liver tissue using CD163 Recombinant antibody (83285-4-RR)

    IF Staining of rat liver using 83285-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using CD163 antibody (83285-4-RR, Clone: 240233A3 ) at dilution of 1:200 and Rhodamine (TRITC)-conjugated Goat Anti-Rabbit IgG(H+L) (SA00007-2), CD20 antibody (31909-1-AP, Magenta), EPCAM/CD326 antibody (32189-1-AP, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat small intestine tissue using CD163 Recombinant antibody (83285-4-RR)

    IF Staining of rat small intestine using 83285-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat small intestine tissue using CD163 antibody (83285-4-RR, Clone: 240233A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83285-4-RR

    Affinity and Kinetic Characterization of 83285-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83285-4-RR against Mouse CD163 were performed. The affinity constant is 2.25 nM.

CD163 Recombinant monoclonal antibody

CD163 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83285-4-RR
Clone No.240233A3

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

240233A3, M130, sCD163

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, mouse lung tissue, rat spleen tissue, rat liver tissue
Positive IHC detected inmouse liver tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat liver tissue, rat small intestine tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83285-4-RR targets CD163 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0794

Product name: Recombinant Mouse CD163 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 39-1045 aa of NM_053094.2

Sequence: VTNAPGEMKKELRLAGGENNCSGRVELKIHDKWGTVCSNGWSMNEVSVVCQQLGCPTSIKALGWANSSAGSGYIWMDKVSCTGNESALWDCKHDGWGKHNCTHEKDAGVTCSDGSNLEMRLVNSAGHRCLGRVEIKFQGKWGTVCDDNFSKDHASVICKQLGCGSAISFSGSAKLGAGSGPIWLDDLACNGNESALWDCKHRGWGKHNCDHAEDVGVICLEGADLSLRLVDGVSRCSGRLEVRFQGEWGTVCDDNWDLRDASVVCKQLGCPTAISAIGRVNASEGSGQIWLDNISCEGHEATLWECKHQEWGKHYCHHREDAGVTCSDGADLELRLVGGGSRCAGIVEVEIQKLTGKMCSRGWTLADADVVCRQLGCGSALQTQAKIYSKTGATNTWLFPGSCNGNETTFWQCKNWQWGGLSCDNFEEAKVTCSGHREPRLVGGEIPCSGRVEVKHGDVWGSVCDFDLSLEAASVVCRELQCGTVVSILGGAHFGEGSGQIWGEEFQCSGDESHLSLCSVAPPLDRTCTHSRDVSVVCSRYIDIRLAGGESSCEGRVELKTLGAWGPLCSSHWDMEDAHVLCQQLKCGVAQSIPEGAHFGKGAGQVWSHMFHCTGTEEHIGDCLMTALGAPTCSEGQVASVICSGNQSQTLLPCSSLSPVQTTSSTIPKESEVPCIASGQLRLVGGGGRCAGRVEVYHEGSWGTVCDDNWDMTDANVVCKQLDCGVAINATGSAYFGEGAGAIWLDEVICTGKESHIWQCHSHGWGRHNCRHKEDAGVICSEFMSLRLTNEAHKENCTGRLEVFYNGTWGSIGSSNMSPTTVGVVCRQLGCADNGTVKPIPSDKTPSRPMWVDRVQCPKGVDTLWQCPSSPWKQRQASPSSQESWIICDNKIRLQEGHTDCSGRVEIWHKGSWGTVCDDSWDLNDAKVVCKQLGCGQAVKALKEAAFGPGTGPIWLNEIKCRGNESSLWDCPAKPWSHSDCGHKEDASIQCLPKMTSESHHGTGHPT

相同性解析による交差性が予測される生物種
Full Name CD163 antigen
Calculated molecular weight121 kDa
Observed molecular weight160-170 kDa
GenBank accession numberNM_053094.2
Gene Symbol Cd163
Gene ID (NCBI) 93671
RRIDAB_3670952
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ2VLH6-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

Protocols

Product Specific Protocols
IF protocol for CD163 antibody 83285-4-RRDownload protocol
IHC protocol for CD163 antibody 83285-4-RRDownload protocol
WB protocol for CD163 antibody 83285-4-RRDownload protocol
Standard Protocols
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