Validation Data Gallery

Filter: AllWBIPIHCIF-P

WB analysis of human placenta using 68218-1-Ig (same clone as 68218-1-PBS)
human placenta tissue were subjected to SDS PAGE followed by western blot with 68218-1-Ig (CD163 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.
IP experiment of human placenta using 68218-1-Ig (same clone as 68218-1-PBS)
IP result of anti-CD163 (IP:68218-1-Ig, 4ug; Detection:68218-1-Ig 1:4000) with human placenta tissue lysate 1160 ug. This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 68218-1-Ig (same clone as 68218-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 68218-1-Ig (same clone as 68218-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 68218-1-Ig (same clone as 68218-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 68218-1-Ig (same clone as 68218-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 68218-1-Ig (same clone as 68218-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 68218-1-Ig (same clone as 68218-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 68218-1-Ig (same clone as 68218-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 68218-1-Ig (same clone as 68218-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68218-1-Ig (CD163 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.
IF Staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CD163 antibody (68218-1-Ig, Clone: 2G7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.
IF Staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CD163 antibody (68218-1-Ig, Clone: 2G7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68218-1-PBS in a different storage buffer formulation.

CD163 Monoclonal antibody, PBS Only

CD163 Monoclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Cat No. 68218-1-PBS

Clone No.2G7A5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

Scavenger receptor cysteine-rich type 1 protein M130, MM130, M130, Hemoglobin scavenger receptor, CD163 molecule

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68218-1-PBS targets CD163 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag26721 Product name: Recombinant human CD163 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 46-401 aa of BC051281 Sequence: GTDKELRLVDGENKCSGRVEVKVQEEWGTVCNNGWSMEAVSVICNQLGCPTAIKAPGWANSSAGSGRIWMDHVSCRGNESALWDCKHDGWGKHSNCTHQQDAGVTCSDGSNLEMRLTRGGNMCSGRIEIKFQGRWGTVCDDNFNIDHASVICRQLECGSAVSFSGSSNFGEGSGPIWFDDLICNGNESALWNCKHQGWGKHNCDHAEDAGVICSKGADLSLRLVDGVTECSGRLEVRFQGEWGTICDDGWDSYDAAVACKQLGCPTAVTAIGRVNASKGFGHIWLDSVSCQGHEPAVWQCKHHEWGKHYCNHNEDAGVTCSDGSDLELRLRGGGSRCAGTVEVEIQRLLGKVCDRG 相同性解析による交差性が予測される生物種
Full Name	CD163 molecule
Calculated molecular weight	1156 aa, 125 kDa
Observed molecular weight	145-150 kDa
GenBank accession number	BC051281
Gene Symbol	CD163
Gene ID (NCBI)	9332
ENSEMBL Gene ID	ENSG00000177575
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q86VB7
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

CD163 is a transmembrane protein which belongs to the scavenger receptor cysteine-rich (SRCR) superfamily. This protein is a scavenger receptor for the hemoglobin-haptoglobin complex and is a marker for monocytes and macrophages. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human placenta using 68218-1-Ig (same clone as 68218-1-PBS)

IP experiment of human placenta using 68218-1-Ig (same clone as 68218-1-PBS)

IHC staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)

IHC staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)

IHC staining of human placenta using 68218-1-Ig (same clone as 68218-1-PBS)

IHC staining of human placenta using 68218-1-Ig (same clone as 68218-1-PBS)

IHC staining of human tonsillitis using 68218-1-Ig (same clone as 68218-1-PBS)

IHC staining of human tonsillitis using 68218-1-Ig (same clone as 68218-1-PBS)

IHC staining of human lung cancer using 68218-1-Ig (same clone as 68218-1-PBS)

IHC staining of human lung cancer using 68218-1-Ig (same clone as 68218-1-PBS)

IHC staining of human appendicitis using 68218-1-Ig (same clone as 68218-1-PBS)

IHC staining of human appendicitis using 68218-1-Ig (same clone as 68218-1-PBS)

IHC staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)

IF Staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)

IF Staining of human liver using 68218-1-Ig (same clone as 68218-1-PBS)

CD163 Monoclonal antibody, PBS Only

CD163 Monoclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information