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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD157 Recombinant antibody (84417-5-RR)

    WB analysis using 84417-5-RR (same clone as 84417-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84417-5-RR (CD157 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84417-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD157 Recombinant antibody (84417-5-RR)

    IHC staining of mouse spleen using 84417-5-RR (same clone as 84417-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84417-5-RR (CD157 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84417-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CD157 Recombinant antibody (84417-5-RR)

    IF Staining of RAW 264.7 using 84417-5-RR (same clone as 84417-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD157 antibody (84417-5-RR, Clone: 241822F10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84417-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84417-5-RR

    Affinity and Kinetic Characterization of 84417-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84417-5-RR against Mouse CD157 were performed. The affinity constant is 5.36 nM.

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Mouse CD157 Recombinant antibody, PBS Only

CD157 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84417-5-PBS
Clone No.241822F10

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Bp3, Bone marrow stromal antigen 1, Antigen BP3, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2, ADP-ribosyl cyclase 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84417-5-PBS targets CD157 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name bone marrow stromal cell antigen 1
Calculated molecular weight35kDa
Observed molecular weight40-46 kDa
Gene Symbol Bst1
Gene ID (NCBI) 12182
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ64277
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CD157 is a glycosyl phosphotidylinositol-anchored protein that stimulates pre-B-cell growth and has adenosine diphosphate (ADP)-ribosyl cyclase and cyclic ADP-ribose (cADPR) hydrolase activity (PMID: 9473467, 11866528). The two enzymatic activities are responsible for the synthesis and hydrolysis of cADPR, a novel second messenger of calcium release from intracellular calcium stores. CD157 has a calculated molecular mass of 30-36 kDa and an apparent molecular mass of 40-46 kDa due to glycosylation (PMID: 8941363, 10491089).