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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD147 Polyclonal antibody (32187-1-AP)

    WB analysis using 32187-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32187-1-AP (CD147 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CD147 Polyclonal antibody (32187-1-AP)

    IHC staining of mouse liver using 32187-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32187-1-AP (CD147 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using CD147 Polyclonal antibody (32187-1-AP)

    IHC staining of mouse small intestine using 32187-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 32187-1-AP (CD147 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using CD147 Polyclonal antibody (32187-1-AP)

    IHC staining of mouse small intestine using 32187-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 32187-1-AP (CD147 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CD147 Polyclonal antibody (32187-1-AP)

    IHC staining of mouse liver using 32187-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32187-1-AP (CD147 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CD147 Polyclonal antibody

CD147 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32187-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

Basic immunoglobulin superfamily, Basigin, Bsg, HT7 antigen, Membrane glycoprotein gp42

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRAW 264.7 cells, mouse small intestine tissue, mouse spleen tissue, mouse thymus tissue
Positive IHC detected inmouse small intestine tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32187-1-AP targets CD147 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name basigin
Calculated molecular weight42kDa
Observed molecular weight35-55 kDa
GenBank accession numberNM_009768.2
Gene Symbol CD147
Gene ID (NCBI) 12215
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP18572-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion, an extracellular portion, and a single transmembrane region. CD147 is expressed in various cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte, and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported thatthe spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020).

Protocols

Product Specific Protocols
WB protocol for CD147 antibody 32187-1-APDownload protocol
IHC protocol for CD147 antibody 32187-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols