Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HUVEC cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
| FC | See 3 publications below |
Product Information
65181-1-Ig targets CD146/MCAM in WB, IF/ICC, FC applications and shows reactivity with human, mouse, rabbit, canine samples.
| Tested Reactivity | human, mouse, rabbit, canine |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
cultured human umbilical cells 相同性解析による交差性が予測される生物種 |
| Full Name | melanoma cell adhesion molecule |
| Calculated molecular weight | 646 aa, 72 kDa |
| GenBank accession number | BC056418 |
| Gene Symbol | CD146 |
| Gene ID (NCBI) | 4162 |
| RRID | AB_2881483 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Purified by protein-A affinity chromatography |
| UNIPROT ID | P43121 |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD146/MCAM antibody 65181-1-Ig | Download protocol |
| IF protocol for CD146/MCAM antibody 65181-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Nrf2 Activation Is Involved in Cyclic Mechanical Stress-Stimulated Osteogenic Differentiation in Periodontal Ligament Stem Cells via PI3K/Akt Signaling and HO1-SOD2 Interaction. | ||
Stem Cell Res Ther Menstrual blood-derived endometrial stem cells alleviate neuroinflammation by modulating M1/M2 polarization in cell and rat Parkinson's disease models | ||
Front Pharmacol CD146 promotes resistance of NSCLC brain metastases to pemetrexed via the NF-κB signaling pathway | ||
Biochim Biophys Acta Mol Cell Res Senolytic elimination of senescent cells improved periodontal ligament stem cell-based bone regeneration partially through inhibiting YAP |
