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Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using CD138/Syndecan-1 Monoclonal antibody (60185-1-Ig)

    WB analysis of BxPC-3 using 60185-1-Ig (same clone as 60185-1-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 60185-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60185-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50044-1

    Sandwich ELISA standard curve of MP50044-1

    Sandwich ELISA standard curve of MP50044-1, SDC1,CD138 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60185-1-PBS. Detection antibody: 67155-1-PBS. Standard: Ag17733. Range: 0.188-12 ng/mL.

  • Sample test of MP50044-1

    Sample test of MP50044-1

    Plasma of sixteen individual healthy human donors was measured. The CD138/Syndecan-1 concentration of detected samples was determined to be 293.79 ng/mL with a range of 89.65-474.60 ng/mL.

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This product has been discontinued. We recommend 67155-1-Ig as an alternative.
For more information, please contact us.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60185-1-PBS targets CD138/Syndecan-1 as part of a matched antibody pair:

MP50044-1: 60185-1-PBS capture and 67155-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD138/Syndecan-1 fusion protein Ag0823 相同性解析による交差性が予測される生物種
Full Name syndecan 1
Calculated molecular weight 32.5 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC008765
Gene Symbol CD138
Gene ID (NCBI) 6382
RRIDAB_10859833
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP18827
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Syndecan-1 (SDC1), also named CD138, is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. SDC1 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.