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Tested Applications
| Positive FC detected in | human peripheral blood mononuclear cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 10 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
PE-65159 targets CD137 in FC applications and shows reactivity with Human, Non-human primates samples.
| Tested Reactivity | Human, Non-human primates |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Recombinant human CD137 ectodomain 相同性解析による交差性が予測される生物種 |
| Full Name | tumor necrosis factor receptor superfamily, member 9 |
| Calculated molecular weight | 28 kDa |
| GenBank accession number | BC006196 |
| Gene Symbol | TNFRSF9/CD137 |
| Gene ID (NCBI) | 3604 |
| RRID | AB_2883937 |
| Conjugate | PE Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 496 nm, 565 nm / 578 nm |
| Form | |
| Form | Liquid |
| Purification Method | The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
| UNIPROT ID | Q07011 |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PE CD137 antibody PE-65159 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |