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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD134/OX40 Recombinant antibody (84247-4-RR)

    WB analysis using 84247-4-RR (same clone as 84247-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84247-4-RR (OX40/TNFRSF4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84247-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84247-4-RR

    Affinity and Kinetic Characterization of 84247-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84247-4-RR against Human CD134/OX40 were performed. The affinity constant is 5.21 nM.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CD134/OX40 Recombinant antibody, PBS Only

CD134/OX40 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 84247-4-PBS
Clone No.241398B4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

TNFRSF4, OX40L receptor, OX40, CD134, ACT35 antigen

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84247-4-PBS targets CD134/OX40 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 4
Calculated molecular weight 29 kDa
Observed molecular weight45-50 kDa
GenBank accession numberNM_003327
Gene Symbol CD134
Gene ID (NCBI) 7293
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP43489
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CD134 (OX40) is a member of the TNFR-superfamily of receptors. Predominantly expressed on activated T cells, CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule. CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmunity (PMID: 26215166). CD134 can interact with TRAF2, TRAF3, and TRAF5.