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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD122/IL-2RB Polyclonal antibody (32546-1-AP)

    WB analysis using 32546-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 32546-1-AP (CD122/IL-2RB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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CD122/IL-2RB Polyclonal antibody

CD122/IL-2RB Polyclonal Antibody for WB, ELISA
Cat No. 32546-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

IL-2RB, Il2rb, IL-2R subunit beta, IL-2 receptor subunit beta, High affinity IL-2 receptor subunit beta

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse spleen tissue, mouse thymus tissue, rat spleen tissue, rat thymus tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32546-1-AP targets CD122/IL-2RB in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name interleukin 2 receptor, beta chain
Calculated molecular weight61 kDa
Observed molecular weight70 kDa
GenBank accession numberNM_008368.4
Gene Symbol Il2rb
Gene ID (NCBI) 16185
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP16297
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to the ability to bind interleukin 2. The low-affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. The beta subunit (IL2RB, also known as CD122 and p75) is involved in receptor-mediated endocytosis and transduces the mitogenic signals of interleukin 2.

Protocols

Product Specific Protocols
WB protocol for CD122/IL-2RB antibody 32546-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols