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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD11c (3.9) Mouse IgG1 Recombinant Antibody

CD11c Recombinant Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

3.9

Cat no : 65511-1-MR

Synonyms

CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6

Validation Data Gallery

Applications:
  • FC experiment of human PBMCs using 65511-1-MR

    FC experiment of human PBMCs using 65511-1-MR

    1x10^6 human PBMCs were surface stained with 0.4 ug Anti-Human CD11c Mouse Recombinant Antibody (65511-1-MR, Clone:3.9) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with FITC-conjugated Anti-Human CD14. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

  • FC experiment of human PBMCs using 65511-1-MR

    FC experiment of human PBMCs using 65511-1-MR

    1x10^6 human PBMCs were surface stained with 0.4 ug Anti-Human CD11c Mouse Recombinant Antibody (65511-1-MR, Clone:3.9) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

  • FC experiment of human PBMCs using 65511-1-MR

    FC experiment of human PBMCs using 65511-1-MR

    1x10^6 human PBMCs were surface stained with 0.4 ug Anti-Human CD11c Mouse Recombinant Antibody (65511-1-MR, Clone:3.9) (left and red) or 0.4 ug Anti-Human CD11c Mouse Monoclonal Antibody (Clone:3.9) (center and green), and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with FITC-conjugated Anti-Human CD14. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

Tested Applications

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD11c Ready-To-Use IHC Kit. CD11c primary antibody included.

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65511-1-MR targets CD11c in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Recombinant
Type Antibody
Immunogen Rheumatoid synovial cells and human monocytes 相同性解析による交差性が予測される生物種
Full Name integrin, alpha X (complement component 3 receptor 4 subunit)
Calculated molecular weight 1169 aa, 129 kDa
GenBank accession numberBC038237
Gene symbol ITGAX
Gene ID (NCBI) 3687
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).

Protocols

Product Specific Protocols
FC protocol for CD11c antibody 65511-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols