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Validation Data Gallery

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  • Western Blot (WB) analysis of Ramos cells using CD11a/Integrin Alpha L Monoclonal antibody (66256-1-Ig)

    WB analysis of Ramos using 66256-1-Ig (same clone as 66256-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 66256-1-Ig (CD11a/Integrin Alpha L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD11a/Integrin Alpha L Monoclonal antibody (66256-1-Ig)

    IHC staining of human tonsillitis using 66256-1-Ig (same clone as 66256-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66256-1-Ig (CD11a/Integrin alpha-L antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD11a/Integrin Alpha L Monoclonal antibody (66256-1-Ig)

    IHC staining of human tonsillitis using 66256-1-Ig (same clone as 66256-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66256-1-Ig (CD11a/Integrin alpha-L antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD11a/Integrin Alpha L Monoclonal antibody (66256-1-Ig)

    IF Staining of human tonsillitis using 66256-1-Ig (same clone as 66256-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11a/Integrin Alpha L antibody (66256-1-Ig, Clone: 1E3H12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD11a/Integrin Alpha L Monoclonal antibody (66256-1-Ig)

    IF Staining of human tonsillitis using 66256-1-Ig (same clone as 66256-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11a/Integrin Alpha L antibody (66256-1-Ig, Clone: 1E3H12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CD11a/Integrin Alpha L Monoclonal antibody (66256-1-Ig)

    IF Staining of Jurkat using 66256-1-Ig (same clone as 66256-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD11a/Integrin Alpha L antibody (66256-1-Ig, Clone: 1E3H12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66256-1-PBS in a different storage buffer formulation.

CD11a/Integrin Alpha L Monoclonal antibody, PBS Only

CD11a/Integrin Alpha L Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 66256-1-PBS
Clone No.1E3H12

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

CD11a, 1E3H12, CD11 antigen-like family member A, Integrin alpha L, Integrin alpha-L

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66256-1-PBS targets CD11a/Integrin Alpha L in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7813

Product name: Recombinant human CD11a/Integrin alpha-L protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 25-350 aa of BC008777

Sequence: SYNLDVRGARSFSPPRAGRHFGYRVLQVGNGVIVGAPGEGNSTGSLYQCQSGTGHCLPVTLRGSNYTSKYLGMTLATDPTDGSILFAAVQFSTSYKTEFDFSDYVKRKDPDALLKHVKHMLLLTNTFGAINYVATEVFREELGARPDATKVLIIITDGEATDSGNIDAAKDIIRYIIGIGKHFQTKESQETLHKFASKPASEFVKILDTFEKLKDLFTELQKKIYVIEGTSKQDLTSFNMELSSSGISADLSRGHAVVGAVGAKDWAGGFLDLKADLQDDTFIGNEPLTPEVRAGYLGYTVTWLPSRQKTSLLASGAPRYQHMGRV

相同性解析による交差性が予測される生物種
Full Name integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
Calculated molecular weight 129 kDa
GenBank accession numberBC008777
Gene Symbol CD11a
Gene ID (NCBI) 3683
ENSEMBL Gene IDENSG00000005844
RRIDAB_2881644
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP20701
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).