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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Human CD11a (TS2/4) Rabbit Recombinant Antibody

CD11a Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

TS2/4

Cat no : CL647-65518

Synonyms

CD11A, Integrin alpha L, ITGAL, LFA 1, LFA 1A, LFA1A

Validation Data Gallery

Applications:
  • FC experiment of human PBMCs using CL647-65518

    FC experiment of human PBMCs using CL647-65518

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD11a Rabbit Recombinant Antibody (CL647-65518, Clone:TS2/4) or CoraLite® Plus 647-conjugated Rabbit IgG control PolyAb (CL647-30000). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL647-65518

    FC experiment of human PBMCs using CL647-65518

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD11a Rabbit Recombinant Antibody (CL647-65518, Clone:TS2/4) or CoraLite® Plus 647-conjugated Rabbit IgG control PolyAb (CL647-30000). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL647-65518

    FC experiment of human PBMCs using CL647-65518

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD11a Rabbit Recombinant Antibody (CL647-65518, Clone:TS2/4) or 5 ul CoraLite® Plus 647 Anti-Human CD11a Mouse Monoclonal Antibody (CL647-65194, Clone:TS2/4). Cells were not fixed. Lymphocytes were gated.

Tested Applications

Positive FC detected inhuman PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65518-1-PBS

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL647-65518 targets CD11a in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HLA-DR CTL line 相同性解析による交差性が予測される生物種
Full Name integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
Calculated molecular weight 129 kDa
GenBank accession numberBC008777
Gene symbol ITGAL
Gene ID (NCBI) 3683
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CD11a antibody CL647-65518Download protocol
Standard Protocols
Click here to view our Standard Protocols